10x PBS Buffer (1 L)
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10x PBS Buffer (1 L)
Each 10X PBS solution is ready to use upon dilution to the desired concentration.
10x PBS Buffer (1 L)
منتج #: 90238222

10x PBS Buffer (1 L)

99% من المشترين سيوصون بهذا المنتج لصديق

منتج #: 90238222

XAF 34415

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Each 10X PBS solution is ready to use upon dilution to the desired concentration.
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مايفيد

Concentration
The 10x PBS Buffer offers a high concentration solution, ensuring reliable dilutions for various biological applications, thereby saving time and reducing the need for frequent preparation.
Versatility
Ideal for cell culture, immunoassays, and molecular biology, this buffer is versatile enough to meet diverse laboratory needs, enhancing overall experimental efficiency.
Convenient Packaging
Packaged in a 1-liter bottle for ease of use, this 10x PBS Buffer reduces handling time and minimizes contamination risk, ensuring a consistent supply for all your research activities.

تفاصيل المنتج

Shop 10x PBS Buffer (1 L) online at a best price in Chad. B0DJCP1GGX
Brand NameGeneric
ManufacturerBioland Scientific LLC
Model NumberPBS01-1L
Part NumberPBS01-1L
Included Componentsbuffer
Item Weight2.2 lbs (1 kg)

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Suitable For
  • Molecular Biologists

    Ideal for molecular biology applications such as dilution, washing, and buffer for various experiments.

  • Cell Culture Users

    Supports cell culture by maintaining osmotic balance and providing essential ions for cell health.

  • Immunologists

    Useful in immunological assays like ELISA and for diluting antibodies, ensuring reliable results.

Not Suitable For
  • Field Researchers

    Not ideal for field applications where stable conditions and lab-grade reagents are unavailable.

وصف المنتج

10x PBS Buffer (1 L)

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أسئلة العملاء & الإجابات

  • سؤال: What is 10x PBS Buffer used for?

    إجابه: 10x PBS Buffer is primarily used in biological and biochemical research as a buffering agent. It helps maintain a stable pH level when diluting samples, making it ideal for cell culture, the dilution of proteins, and washing of cells. Its composition of sodium phosphate, sodium chloride, and potassium chloride provides ionic strength, which is crucial for assays, such as Western blots and ELISA. For example, when preparing cell cultures, using PBS ensures that the cells remain healthy and viable for experimentation.
  • سؤال: How do you prepare a working solution from 10x PBS Buffer?

    إجابه: To prepare a working solution from 10x PBS Buffer, simply dilute it with distilled water. For most applications, a common dilution used is 1 part 10x PBS to 9 parts water, resulting in a 1x PBS solution. Make sure to mix thoroughly. This dilution is ready for use in applications such as washing cells before experiments or as a diluent for biological samples. This preparation process is straightforward, ensuring that you can have a consistent buffer for various experimental procedures.
  • سؤال: Is 10x PBS Buffer suitable for mammalian cell culture?

    إجابه: Yes, 10x PBS Buffer is highly suitable for mammalian cell culture. It provides the necessary ionic environment to stabilize the cells during various biological processes. In cell culture, PBS is often used for rinsing the cells, removing media, or resuspending them without causing damage. As a result, it ensures that cells remain in optimal condition for experiments and assays, enhancing the reliability of your results.
  • سؤال: What is the difference between 1x PBS and 10x PBS?

    إجابه: The main difference between 1x PBS and 10x PBS lies in their concentration. 10x PBS is ten times more concentrated than the 1x formulation. The 10x PBS buffer is typically diluted before use to achieve the desired concentration of 1x, which is the standard working concentration for many laboratory applications. For instance, if you're conducting an experiment that requires a washing step, you’ll use the diluted 1x PBS to ensure optimal pH and ionic strength without affecting your results.
  • سؤال: Can 10x PBS Buffer be used for protein storage?

    إجابه: While 10x PBS Buffer is excellent for preparing solutions, it is not ideal for long-term protein storage. Proteins are generally more stable in solutions that contain stabilizers such as glycerol or specific buffer conditions tailored for the protein's stability. Instead, 10x PBS can be used to dilute proteins for immediate use in assays or experiments. Always consider the nature of your protein and consult relevant literature to choose the best storage conditions.
  • سؤال: How should 10x PBS Buffer be stored?

    إجابه: 10x PBS Buffer should be stored at room temperature or in a refrigerator, depending on the specific formulation and any added components. Avoid exposure to extreme conditions that might alter its pH or ionic concentrations. Once opened, it’s essential to keep it tightly sealed to prevent contamination. Proper storage ensures that the buffer remains effective for various laboratory applications, thus maintaining the integrity of your experimental results.
  • سؤال: Is 10x PBS Buffer toxic?

    إجابه: 10x PBS Buffer is generally considered non-toxic and safe for handling in laboratory environments. However, standard laboratory safety protocols should always be followed. Use gloves and goggles when handling any chemical solutions to protect yourself from potential irritants. Despite its safety profile, awareness of proper disposal methods and material safety data sheets (MSDS) for the components used in PBS can ensure safe handling practices.
  • سؤال: What are the typical applications of 10x PBS Buffer in research?

    إجابه: 10x PBS Buffer is utilized in a wide range of research applications, including cell washing, sample dilution, and protein assays. It is commonly used in immunological techniques, such as ELISA and Western blots, where maintaining a stable pH and ionic strength is crucial. Additionally, it's indispensable in the preparation and dilution of biological samples for microscopy and flow cytometry. This versatility makes 10x PBS a fundamental tool in many research laboratories.
  • سؤال: Can I use 10x PBS Buffer with live cell imaging?

    إجابه: Yes, 10x PBS Buffer can be used for live cell imaging; however, it must first be diluted to a 1x concentration to avoid hyperosmolarity that could affect cell health. In live cell imaging experiments, 1x PBS can serve as a wash buffer before adding imaging media. Its balanced ionic strength helps maintain cell integrity during microscopy, making it suitable for various imaging techniques, including fluorescence and confocal microscopy.
  • سؤال: Where can I buy 10x PBS Buffer (1 L) in Chad?

    إجابه: You can buy 10x PBS Buffer (1 L) from Ubuy in Chad. Ubuy offers a wide selection of laboratory reagents and supplies, making it easy to find quality products like 10x PBS. So, if you're looking to purchase this essential buffer, Ubuy is your go-to source for reliable lab supplies.

Buffers PBS01-1L Editorial Review

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مراجعات العملاء وتقييماتهم

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إيجابيات

  • High quality reagents
  • Stable and long shelf life
  • Versatile buffer application
  • Convenient 1 L size
  • Accurate pH maintenance

سلبيات

  • Occasional precipitation issue

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“Wonderful online shopping experience, smooth transaction from the start. Payment method works conveniently and delivery is unexpectedly fast and reliable. You go the extra mile for service. What makes this even more amazing, you deliver to Namibia. I will remain a happy Ubuy customer and will increase my purchases for sure! Thank you!”

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“Very easy to find the products what you need, and so fast delivery, that’s why I highly recommended to others costumers to used ubuy.”

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XAF 34415

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المميزات والفوائد

  • Ready-to-use 10X PBS solution for your laboratory needs.
  • Convenient dilution to 1X working concentration for quick application.
  • Ideal for Western blot, ELISA, and immunoprecipitation.
  • Formulated with ultrapure-grade phosphate buffers and saline solutions.
  • Clear colorless liquid with a pH of 7.4 ± 0.2.
  • Filtered with a 0.45µm filter ensuring high purity.

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